Enhanced emulsan production in mutants of Acinetobacter calcoaceticus RAG-1 selected for resistance to cetyltrimethylammonium bromide
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چکیده
منابع مشابه
Role for Emulsan in Growth of Acinetobacter calcoaceticus RAG-1 on Crude Oil.
When Acinetobacter calcoaceticus RAG-1 was grown together with an emulsan-deficient mutant on crude oil, only the emulsan-producing RAG-1 was found to grow, regardless of whether the medium was supplemented with emulsan. The results suggested that the cell-associated form of the bioemulsifier is the biologically active species required for growth on crude oil. A revertant of an emulsan-deficien...
متن کاملEmulsan production by Acinetobacter calcoaceticus in the presence of chloramphenicol.
When exponentially growing cultures of Acinetobacter calcoaceticus RAG-1 or RAG-92 were either treated with inhibitors of protein synthesis or starved for a required amino acid, there was a stimulation in the production of emulsan, an extracellular polyanionic emulsifier. Emulsan synthesis in the presence of chloramphenicol was dependent on utilizable sources of carbon and nitrogen and was inhi...
متن کاملSelf-Cycling Fermentation Applied to Acinetobacter calcoaceticus RAG-1.
The self-cycling fermentation (SCF) technique was applied to a culture of Acinetobacter calcoaceticus RAG-1. This method was shown to result in synchronization of the cells, achieving a 77% improvement in cell synchrony over that of the batch case. Cellular occurrences, averaged out by asynchronous batch cultures, were magnified by the temporal alignment of metabolic events brought about by the...
متن کاملThe isolation of cell surface mutants of Acinetobacter calcoaceticus RAG-1.
The isolation of cell surface mutants of Acinetobacter calcoaceticus RAG-1 was demonstrated by a method based on the differential immunoprecipitating properties of wild-type cells and mutants. In the presence of antiserum, about 99% of wild-type cells in suspension sedimented following overnight agglutination. The kinetics of antiserum-induced agglutination was quantified by measuring the time-...
متن کاملAdjuvant activity of emulsan, a secreted lipopolysaccharide from acinetobacter calcoaceticus.
Several promising adjuvant candidates have been studied over the past 75 years; however, only alum is currently approved for human use. The complex acylated polysaccharide emulsan, secreted from Acinetobacter calcoaceticus, represents a new candidate. Unique features of this family of polymers are their amenability to structural tailoring and their emulsification behavior. We demonstrate that e...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1986
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.52.1.146-151.1986